Succinate MIP Biosensor (RUO)
The Succinate MIP Biosensor is a highly selective, ultra-sensitive electrochemical sensor engineered for rapid quantification of succinate, a central metabolite in the TCA cycle and a key indicator of mitochondrial function, inflammation, hypoxia, and metabolic stress.
Using Fluidome’s proprietary molecularly imprinted polymer (MIP) technology, this biosensor provides the binding precision of antibodies while offering superior stability, robustness, and cost-effectiveness for metabolic studies.
The succinate sensor integrates seamlessly with standard electrochemical readout systems and can be paired with microfluidic cartridges (MIP-Chip format) for automated sample-to-answer workflows, including plasma separation and redox enhancement.
Detection can be performed directly from blood, serum, or plasma with minimal sample preparation.
Key Features
-
Ultrasensitive detection with a limit of detection (LOD) of 5 nM
-
Wide linear detection range suitable for physiological and pathological succinate levels
-
Rapid 5–10 minute assay time
-
High specificity using a tailor-made MIP “plastic antibody”
-
Compatible with blood, serum, and plasma
-
Works as electrode-only or microfluidic MIP-Chip
-
High batch-to-batch reproducibility (CV <5%)
-
Stable analytical performance under proper storage conditions
Applications
Validated and published research applications include:
-
Quantification of succinate in whole blood or plasma
-
Metabolic profiling in hypoxia, sepsis, mitochondrial dysfunction, and inflammation
-
Whole-blood metabolite detection without preprocessing (MIP-Chip)
-
Organ-on-chip and microfluidic metabolic monitoring
-
Method development for electrochemical biosensing
-
High-throughput metabolite screening
-
Preclinical metabolic disease and immunometabolism research (RUO)
Packaging and Specifications
Packaging material: Polypropylene tube or microfluidic cartridge
Sterility: Not sterilized
Packaging: 1 biosensor per package
Format options: Electrode-only / integrated microfluidic chip
Assay time: 5–10 minutes
Technology: MIP-based electrochemical biosensor
Transduction: SWV, CV, EIS compatible
LOD: 5 nM
Linear range: Nanomolar to micromolar concentrations (validated vs. LC–MS)
Sample types: Blood, serum, plasma
Stability: >90% signal retention after 4 weeks
Reproducibility: Coefficient of variation (CV) < 5%
Shipping conditions: Room temperature
Recommended storage: 4°C (short-term) or refrigeration (long-term)
Regulatory status: Research Use Only (RUO)—not for diagnostic use
Analytical Performance
Evidence from published and internal studies demonstrates:
-
Accurate succinate quantification strongly correlated with LC–MS benchmarks
-
Nanomolar sensitivity enabling early metabolic event detection
-
Excellent selectivity against structurally related TCA intermediates
-
Robust signal stability and reproducibility across devices
-
Consistent performance in whole-blood integration via MIP-Chip
-
Stable performance across repeated runs and long-term storage
Technical Note
A high-affinity MIP-based electrochemical biosensor enabling rapid, nanomolar-level, and reproducible quantification of succinate in blood-derived samples. Designed for studies of metabolism, hypoxia, sepsis, mitochondrial dysfunction, and inflammatory pathways.
Important Notes
-
-
The Succinate Biosensor is Research Use Only (RUO) and not validated for clinical diagnosis.
-
Bulk orders and multi-analyte biosensor bundles are available.
-
Custom microfluidic integration and cartridge design are available on request.
-
Please contact us for bulk pricing, quotations, or distributor inquiries.
-
