The Crosstalker Plate is both a microbial culture plate and metabolite sampling platform combined into one simple device. Designed to assess the metabolic relationships which occur between two or more species of microbes, the Crosstalker Plate is an essential tool for cutting-edge research on microbial communities where it can uniquely help determine the metabolic contributions of individual members to a complex environment. The Crosstalker Plate allows users to easily sample metabolites that are produced by one species of microbe and consumed by others.
The Crosstalker Plate is designed to enable the measurement of metabolite exchange in polymicrobial communities.
Microbial cells are loaded into the top plate with a 96-well format and are separated from the community metabolite pool by a semi-permeable membrane (6 individual wells/1 community well).
Small molecule metabolites can freely diffuse from the individual wells in the top plate through the Crosstalker Plate’s semi-permeable membranes and can then pass through the community pool (bottom plate) to interact with microbes housed in other individual wells.
Other cell types or biological tissues can also be seeded in the Crosstalker bottom plate.
Tested applications for the Crosstalker Plate include metabolic dynamics in polymicrobial infections.
Material
Polystyrene
Sterility
Ethylene oxide gas sterilized
Packaging
1 platform (top and bottom plate)/package
Size
96-well (top plate)/16-well (bottom plate)
Membrane pore size
50nm, 0.1µm and 0.2 µm, 1µm, 3µm, 5µm
Membrane material
Polycarbonate
Compartment Volume
2.2 mL
Working Volume
100 µL (top plate)/2 mL (bottom plate)
Color
Transparent
Binding Type
Non-treated surface
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